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OnDemand Kits

Guaranteed High Quality Custom Kits Expertly Designed and Developed on Request


Product Features


  • Top quality assays with guaranteed results
  • Designed by ‘in-house’ bioinformatical experts, not databases
  • Scientifically validated in our laboratory
  • Preliminary feasibility confirms if OnDemand assay is likely possible
  • An assay for almost any application

OnDemand Kits are sold for research use only and are not licensed for diagnostic procedures.

 

About This Product


OnDemand Kits are Real-Time PCR assays which we have the capability and capacity to design and develop. They are for ‘hotspot’ target organisms which have particular importance in their individual areas of interest and therefore we understand the importance of developing an assay. The OnDemand Kits can be developed on request and to meet your specific requirements meaning you will be provided with an assay which is of the highest quality and which works for you.

We offer specific qPCR assay development including primer/probe design, PCR mix optimization and assay development to ensure a very effective qPCR reaction. We have already developed a huge range kits as part of our ever expanding genesig catalogue. This service allows for the combination of bioinformatic design and development, in conjunction with laboratory testing and evaluation, to provide personally designed primers to match your exact needs and ensure high levels of sensitivity and specificity.

Upon request of development, one of our expert team members will be in contact shortly to completely understand any specific requirements you may have (incl. exclusivity criteria, etc), and they are happy to talk you through the whole process.

A preliminary investigation of the targets below suggest these organisms can have an effective qPCR assay being developed which would match our high performance criteria of specificity, sensitivity and efficiency.

If you are interested in any of these OnDemand Kits or wish to have a custom kit developed please check out the purchasing section below, or alternatively contact us via email: enquiry@primerdesign.co.uk or telephone: +44 (0)2380 748 830.

 

About / Technical


Our standard assay formats include Double-Dye cleavage probe (e.g. TaqMan® probes) or as primer only kits for use with SYBR green chemistry. If you require something more alternative or sophisticated, please do not hesitate to get in contact to enquire about its feasibility.

Kit Contents

  • OnDemand Advanced Kit: Target primer & probe set, positive control, internal extraction control primer & probe set, internal extraction control template, endogenous control primer & probe set, RNase/DNase free water, template preparation buffer, Feasibility Report, Kit Data Sheet (incl. anchor nucleotide).
  • OnDemand Easy Kit: Target primer & probe set, oasig Lyophilised qPCR Master Mix (DNA or RNA), oasig resuspension buffer, positive control, internal extraction control, RNase/DNase free water, template preparation buffer, 54x genesig q16 reaction tubes, Feasibility Report, Kit Data Sheet (incl. anchor nucleotide).

Ours kits will be supplied packaged, optimized and ready to use. If you are looking for pre-dispensed plates or qPCR arrays please contact us.

 

Resources


OnDemand Advanced Kit Handbook
 
OnDemand Easy Kit Handbook


Purchasing


Price

Price on application - following the process outline below will help us provide you the best price.

Catalogue No.

When ordering an OnDemand Kit, you firstly need to order a Feasibility Report, either before or during the kit development. This is a complete bioinformatic identification of the target regions which are totally specific to your requirements. Below are those two catalogue nos. required.

  • Feasibility Report: OnDemand-Feas
  • Design & Development (150 reaction kit): OnDemand-Dev

Process Outline

If you are interested any of the organisms below, or on any organism not listed below or in our catalogue, the process is as follows:

  • Download the Design Request Form above, or alternatively this can be emailed on request. 
  • This will help us understand the specific design requirements and specifications
  • Attach the form and email us on enquiry@primerdesign.co.uk
  • Following receipt of the completed form, our expert Research & Development team will review the request and we aim to respond within 1 working day
  • The service timelines, costings and development process details will be supplied


FAQ


What do you get at the end of an OnDemand Kit Development?

  1. Validated, optimized and effective qPCR kit
  2. Product datasheet (incl. anchor nucleotide)
  3. Feasibility report
  4. Handbook

How long does the kit development take?
It should take 4-6 weeks per kit for design and development.

Can you order recurrent kits of the same design?
Absolutely, with significant cost reduction and dispatched with days.

What info do you need to design my kit?
Typically a gene name, species, and the NCBI accession number is required.

I’m working on an unusual species. Can you still help?
Yes. If you are working on an unusual species then we will check that good quality sequence data is available. If not we may be able to work from sequence data from a closely related species. Once synthesized, we can then validate on cDNA sent in by you, or if that is not possible then we will work on a synthetic version of the amplicon to validate the performance of the primers.

I’m working on a virus/bacteria/pathogen species. Can you design primers for these?
Yes. We have over 600 genesig pathogen detection kits already available. If your pathogen of interest is not in the list we can develop a new kit for you from scratch. You can check for your target here.

What qPCR machine(s) will these kits work on?
Easy Kit Format: Designed for optimum performance with the genesig q16 and only recommended to be used with this platform. Advanced Kit Format: These kits are designed to be open platform and will work perfectly on any qPCR machine.

How efficient is the priming of your kit?
We guarantee an efficiency of greater than 90%. 

Are your kit primers intron spanning?
Not always: As standard we will design the very best possible primers for the organism of interest regardless of intron/exon boundaries. We do not design primers to be intron spanning as standard as this is restrictive and the most efficient location for the primers may not be on an exon-exon junction. However, if you require your assays to span an intron please let us know and we will design primers to accommodate your request. If detection of gDNA is a concern we recommend the use of Precision DNase to remove contaminating gDNA from your RNA.

Is the kit guaranteed to be specific?
Yes. Thorough analysis is carried out during the design phase to ensure specificity to the target. But the gold standard test is carried out in our wet laboratory where we prove specific amplification of a single product on biologically derived cDNA or synthetic template.


Examples of OnDemand Kits

 

A preliminary investigation of the targets below suggest these organisms can have an effective qPCR assay developed which would match our high performance criteria of specificity, sensitivity and efficiency.

Human-Related Targets

Antibiotic Resistance Markers

  • blaCTX-M (beta-lactamases)
  • blaCTX-M2/M9 (beta-lactamases)
  • blaSHV
  • blaTEM
  • MecA
  • VanA
  • VanB

Gastrointestinal Infections

  • Aeromonas hydrophila (Bacteria Hemorrhagic Septicemia) (A.hydrophila)
  • Ancylostoma duodenale (A.duodenale)
  • Balamuthia mandrillaris (B.mandrillaris)
  • Bifidobacterium bifidum (B.bifidum)
  • Blastocystis hominis (B.hominis)
  • Clostridium botulinum toxin A (C.botulinum_toxA)
  • Clostridium botulinum toxin B (C.botulinum_toxB)
  • Clostridium botulinum toxin D (C.botulinum_toxD)
  • Clostridium botulinum toxin E (C.botulinum_toxE)
  • Clostridium botulinum toxin F (C.botulinum_toxF)
  • Cryptococcus gattii (C.gattii)
  • Cyclospora cayetanensis (C.cayetanensis)
  • Escherichia coli O104:H4 (E.coli O104:H4)
  • Enterococcus caseliflavus (E.caseliflavus)
  • Enterohemorrhagic Escherichia coli (EHEC)
  • Enteroinvasive Escherichia coli (EIEC)
  • Enterotoxigenic Escherichia coli (ETEC)
  • Helicobacter pylori (H.pylori)
  • Human Rotavirus B
  • Human Rotavirus C
  • Vibrio cholerae all subtypes (V.cholerae_subsp)
  • Necator americanus (N.americanus)
  • Parechovirus
  • Salmonella enterica (S.enterica)
  • Shiga toxin (stx1) producing Escherichia coli (E.coli_stx1)
  • Shiga toxin (stx2f) producing Escherichia coli (E.coli_stx2f)
  • Shigella species
  • Vibrio cholerae toxogenic subtypes (V.cholerae)
  • Vibrio species
  • Yersinia enterocolitica (Y.enterocolitica)

Genotyping

  • BRAF (V600E)
  • GABBR2 codon 421
  • IL23R codon 381
  • rs1024610
  • rs1024611
  • rs12979860

Herpes

  • Human Herpesvirus 6A (HHV6A)
  • Human Herpesvirus 6B (HHV6B)

Periodental

  • Ascaris lumbricoides & Ascaris suum (A.lumbricoides & A.suum)
  • Nitrosomonas oligotropha (N.oligotropha)
  • Nitrobacter species
  • Nitrospira species
  • Porphyromonas gingivalis (P.gingivalis)
  • Prevotella intermedia (P.intermedia)
  • Pseudomonas stutzeri (P.stutzeri)
  • Streptococcus mutans (S.mutans)
  • Streptococcus salivarius (S.salivarius)
  • Tannerella forsythia (T.forsythia)

Respiratory Infections

  • Adenovirus Type D (AdVD)
  • Adenovirus Type F&G (AdVF&G)
  • Actinobacillus capsulatus (A.capsulatus)
  • Chlamydophila pneumoniae (C.pneumoniae)
  • Cryptococcus neoformans (C.neoformans)
  • Geosmithia argillacea (G.argillacea)
  • Human Bocavirus 1 (HBoV1)
  • Human Coronavirus 2012 (MERS) (HCoV_2012_V1)
  • Human Coronavirus Group 1b (HCoV_Gp1)
  • Human Coronavirus Group 2a (HCoV_Gp2)
  • Human Influenza A Virus Subtype H3
  • Human Parainfluenza Virus type 4 (HPIV4)
  • Human Parainfluenza Virus type 4A (HPIV4A)
  • Human Parainfluenza Virus type 4B (HPIV4B)
  • Human Polyomavirus 6 (HPyV6)
  • Human Polyomavirus 7 (HPyV7)
  • Human Polyomavirus 9 (HPyV9)
  • Human Rhinovirus Subtype 1B (HRV1B)
  • Human Rhinovirus Subtype 9 (HRV9)
  • Human Rhinovirus Subtype 29 (HRV29)
  • Merkel Cell Polyomavirus (MCPyV)
  • MRSA-Staphylococcal Cassette Chromosome Mec (MRSA_SCC_global)
  • Mycobacterium avium subspecies paratuberculosis (M.avium subsp. paratuberculosis (Map))
  • Mycobacterium tuberculosis complex targets MPB64 and IS6110 (M.tuberculosis_MPB64/IS6110)
  • SARS Coronavirus (Severe Acute Respiratory Syndrome) 
  • Simkania negevensis (S.negevensis)
  • WU Polyomavirus (WUPyV)

Sexually transmitted Infections

  • Haemophilus ducreyi (H.ducreyi)
  • Human Papillomavirus 6 (HPV6)
  • Human Papillomavirus 11 (HPV11)
  • Human Papillomavirus 16 (HPV16)
  • Human Papillomavirus 18 (HPV18)
  • Human Papillomavirus 31 (HPV31)
  • Human Papillomavirus 33 (HPV33)
  • Human Papillomavirus 45 (HPV45)
  • Human Papillomavirus 52 and 52b (HPV52)
  • Human Papillomavirus 58 (HPV58)

Vector-Borne Infections

  • Borrelia afzelii (B.afzelii)
  • Borrelia garinii (B.garinii)
  • Chaoyang virus (ChaoV)
  • Dengue virus type 3 (Dengue_3)
  • Leishmania major (L.major)
  • Leishmania tropica (L.tropica)
  • Plasmodium knowlesi (P.knowlesi)
  • Plasmodium malariae (P.malariae)
  • Plasmodium ovale (P.ovale)
  • St. Louis encephalitis virus (SLEV)
  • Western Equine Encephalomyelitis Virus (WEEV)
  • Yellow Fever Virus (YFV)

Other Human Pathogens

  • Acanthamoeba castellanii (A.castellanii)
  • Aeromonas caviae (A.caviae)
  • Aeromonas sobria (A.sobria)
  • Aggregatibacter actinomycetemcomitans (A.actino)
  • Aureobasidium pullulans (A.pullulans)
  • Influenza A virus subtype H7N9 (avian influenza)
  • Bacillus atrophaeus (B.atrophaeus)
  • Bacillus licheniformis (B.licheniformis)
  • Bacteriophage MS2
  • Bundibugyo Ebola Virus (BDBV)
  • Burkholderia cepacia Complex (All Species) (B.cepacia_complex)
  • Burkholderia pseudomallei (B.pseudomallei)
  • Bussuquara virus (BSQV)
  • Candida auris (C.auris)
  • Carnobacterium maltaromaticum (C.maltaromaticum)
  • Clostridium difficile (toxin A) (C.difficile_toxA)
  • Clostridium sporogenes (C.sporogenes)
  • Clostridium tetani (C.tetani)
  • Corynebacterium diphtheriae (C.diphtheriae)
  • Coniothyrium telephii (C.telephii)
  • Cronobacter sakazakii (C.sakazakii)
  • Dobrava-Belgrade virus (DOBV)
  • Eastern equine encephalitis virus (EEEV)
  • Ebola virus (2014 outbreak) (EBOV_2014)
  • Encephalitozoon species
  • Eukaryota
  • Filifactor alocis (F.alocis)
  • Fusobacterium nucleatum (F.nucleatum)
  • Geobacillus stearothermophilus (G.stearothermophilus)
  • Human Polyomavirus 12 (HPyV12)
  • Ilheus virus (ILHV)
  • KI polyomavirus (KIPyV)
  • Lactobacillus acidophilus (L.acidophilus)
  • Lactobacillus sakei / Lactobacillus curvatus (L.sakie / L.curvatus)
  • Lassa virus Josiah (LASV-Josiah)
  • Legionella longbeachae (L.longbeachae)
  • Leprosy (Mycobacterium leprae) (M. leprae & M.lepromatosis)
  • Malassezia restricta (M.restricta)
  • Marburg virus (MBGV)
  • Mayaro virus (MAYV)
  • Mumps virus
  • Mycoplasma fermentans (M.fermentans)
  • Mycoplasma hominis (M. hominis)
  • Mycoplasma orale (M.orale)
  • Mycoplasma salivarium (M.salivarium)
  • Nipah henipavirus (N.henipavirus)
  • O'nyong-nyong virus (ONNV)
  • Orientia tsutsugamushi (O.tsutsugamushi)
  • Oxalobacter formigenes (O.formigenes)
  • Pasteurella multocida (P.multocida)
  • Reston ebola virus (RESTV)
  • Rickettsia prowazekii (R.prowazekii)
  • Rocio virus (ROCV)
  • Rubella virus
  • Schistosoma haematobium (S.haematobium)
  • Schistosoma mansoni (S.mansoni)
  • Semliki Forest virus (SFV)
  • Sin Nombre Virus (SNV)
  • Spondweni virus (SPOV)
  • Staphylococcus haemolyticus (S.haemolyticus)
  • STL polyomavirus (STLPyV)
  • Streptococcus mitis (S.mitis)
  • Streptococcus oralis (S.oralis)
  • Streptococcus sanguinis (S.sanguinis)
  • Sudan Ebola Virus (SUDV)
  • Tai Forest Ebola Virus (TAFV)
  • Treponema denticola (T.denticola)
  • Tsukamurella inchonensis (T.inchonensis)
  • Usutu virus (USUV)

Veterinary Targets

 

Avian

  • Avian Adenovirus EDS76 Egg Drop Syndrome (EDS)
  • Avian Influenza A H5N8 (H5N8)
  • Avian Influenza A Virus Subtype H6
  • Avian Influenza A Virus Subtype H9
  • Chicken Anemia Virus (CAV)
  • Columbid circovirus (CoCV)
  • Columbid Herpesvirus 1 (CoHV-1)
  • Duck Hepatitis B Virus (DHBV)
  • Fowl adenovirus (FAdV)
  • Fowlpox Virus (FpV)
  • Gallid Herpesvirus 1 (GaHV-1)
  • Infectious Bursal Disease Virus (IBDV)
  • Lovebird (Agapornis) Sexing
  • Mycoplasma iowae (M.iowae)
  • Ornithobacterium rhinotracheale (C.rhinotracheale)
  • Pigeon adenovirus 1 (PiAV-1)

Bovine / Ovium

  • Anaplasma centrale (A.centrale)
  • Anaplasma marginale (A.marginale)
  • Babesia bigemina (B.bigemina)
  • Babesia bovis (B.bovis)
  • Babesia divergens (B.divergens)
  • Bluetongue Virus Serotype 1 (BTV-1)
  • Bluetongue virus (BTV-8)
  • Bovine adenovirus 5/6/8 (BAdV-5/6/8)
  • Bovine Respiratory Corona Virus (BCoV)
  • Bovine ephemeral fever virus (BEFV)
  • Bovine Parainfluenza Virus Type 3 (BPIV-3)
  • Bovine Respiratory Syncytial Virus (BRSV)
  • Corynebacterium pseudotuberculosis (C.pseudotuberculosis)
  • Foot and Mouth Disease Virus (FMDV)
  • Mycoplasma arginini (M.arginine)
  • Mycoplasma mycoides cluster (M.mycoides cluster)
  • Theileria annulate (T.annulata)
  • Theileria Mutans (T.mutans)
  • Theileria parva (T.parva)
  • Trypanosoma evansi (T.evansi)
  • Vesicular stomatitis Virus (VSV)
  • Wesselsbron virus (WESSV)

Canine

  • Angiostrongylus vasorum (A.vasorum)
  • Canine Adenovirus Type 1 (CAdV-1)
  • Canine Adenovirus Type 2 (CAdV-2)
  • Canine coronavirus (CCV)
  • Canine Norovirus (CNV)
  • Canine influenza virus subtype H3N8
  • Canine Influenza virus subtype H3N2
  • Dirofilaria immitis (D.immitis)
  • Echinococcus granulosus (E.granulosus)
  • Microsporum canis (M.canis)
  • Minute Virus of Canines (Canine minute virus) (MVC)
  • Microsporum gypseum (M.gypseum)
  • Mycoplasma species haemofelis and haemocanis (M.haemofelis / M.haemocanis)
  • Porphyromonas gulae (P.gulae)

Equine

  • African horse sickness virus (AHSV)
  • Babesia caballi (B.caballi)
  • Candidatus Mycoplasma turicensis (C.M.turicensis)
  • Equine arteritis virus (EAV)
  • Equid Herpesvirus 3 (EHV-3)
  • Equid Herpesvirus 5 (EHV-5)
  • Equine Infectious Anemia Virus (EIAV)
  • Equine dermatophytosis (Ringworm)
  • Equine Rhinovirus type 1 (ERhV1)
  • Equine Rhinovirus type 2 (ERhV2)
  • Klebsiella pneumoniae capsule type 1 (K.pneumoniae_capsule-1)
  • Klebsiella pneumoniae capsule type 2 (K.pneumoniae_capsule-2)
  • Klebsiella pneumoniae capsule type 5 (K.pneumoniae_capsule-5)
  • Rhodococcus equi (R.equi)
  • Strongylus vulgaris (S.vulgaris)
  • Theileria equi (T.equi)
  • Taylorella equigenitalis (T.equigenitalis) (CEMO)
  • Trypanosoma equiperdum (T.equiperdum)

Piscean

  • Atlantic salmon paramyxovirus (ASPV)
  • Betanodavirus
  • Candidatus Branchiomonas cysticola (C.B.cysticola)
  • Cyprinid Herpesvirus 3 (CyHV-3)
  • Enterocytozoon hepatopenaei (E.hepatopenaei)
  • Epizootic Haematopoietic Necrosis Virus (EHNV)
  • Grass carp reovirus (GCRV)
  • Infectious Hematopoietic Necrosis Virus (IHNV)
  • Infectious Salmon Anaemia Virus (ISAV)
  • Infectious Spleen and Kidney Necrosis Virus (ISKNV)
  • Lymphocystivirus (LCV)
  • Photobacterium damselae (P.damselae)
  • Photobacterium phosphoreum (P.phosphoreum)
  • Piscirickettsia salmonis (P.salmonis)
  • Tilapia Lake Virus (TiLV)
  • Viral Hemorrhagic Septicemia Virus (VHSV)
  • Yersinia ruckeri (Y.ruckeri)

Porcine

  • Brachyspira hyodysenteriae (B.hyodysenteriae)
  • Brucella suis (B.suis)
  • Classical swine fever virus (CSFV)
  • Mycoplasma hyopneumoniae (M.hyopneumoniae)
  • Mycoplasma hyorhinis (M.hyorhinis)
  • Mycoplasma suis (M.suis)
  • Porcine circovirus 1 (PCV1)
  • Suid alphaherpesvirus 1 (SuHV1)
  • Suid betaherpesvirus 2 (SuHV2)
  • Swinepox
  • Transmissible gastroenteritis coronavirus (TGEV)
  • Transmissible Gastroenteritis Virus & Porcine Respiratory Coronavirus (TGEV / PRCV)

Other Veterinary Pathogens

  • Ancylostoma duodenale (A.duodenale)    
  • Aspiculuris tetraptera (A.tetraptera)
  • Batrachochytrium dendrobatidis (B.dendrobatidis)
  • Botrytis cinereal (B.cinerea)
  • Cladosporium spp
  • Clavibacter michiganensis sub-species michiganensis (Cmm)
  • Encephalitozoon cuniculi (E.cuniculi)
  • Epizootic Hemorrhagic Disease Virus (EHDV)
  • Fusarium species
  • Israeli Acute Paralysis Virus (IAPV)
  • Maize chlorotic mottle virus (MCMV)
  • Myobia musculi (M.musculi)
  • Myocoptes musculinus (M.musculinus)
  • Rabbit haemorrhagic disease virus type 2 (RHDV-2)
  • Slow bee paralysis virus (SBPV)
  • Spironucleus muris (Spironucleus muris)
  • Sugarcane Mosaic Virus (SCMV)
  • Syphacia obvelata (S.obvelata)
  • Syphacia muris (S.muris)
  • Vaccinia Virus
  • White spot syndrome virus (WSSV)
  • Vesivirus 2117

Food & Agriculture Targets

Animal Finder

  • Atlantic Cod (Gadus morhua)
  • Cat (Felis silvestris)
  • Coley (Pollachius virens)
  • Deer (Venison)
  • Dog (Canis lupus familiaris)
  • Donkey (Equus africanus asinus)
  • Duck (Anas platyrhynchos)
  • European Plaice (Pleuronectes platessa)
  • Goat (Capra hircus)
  • Haddock (Melanogrammus aeglefinus)
  • Ostrich (Struthio camelus)
  • Pollock (Pollachius pollachius)
  • Universal Fish
  • Water Buffalo (Bubalus bubalis)
  • Whiting (Merlangius merlangus)
  • Wild Turkey (Meleagris gallopavo)

GMO

  • GMO FMV
  • GMO p35s
  • GMO tNOS
  • Maize-Bt176
  • Maize-Bt11
  • Maize MON810
  • Maize NK603
  • Maize p35s & tNOS
  • NOS terminator in GM crops
  • Soya p35s & tNOS
  • Soya RR

Pathogens

  • Bifidobacterium longum (B.longum)
  • Empetrum nigrum (E.nigrum)
  • Lactococcus lactis (L.lactis)
  • Lactobacillus plantarum / Lactobacillus paraplantarum (L.plantarum / L.paraplantarum)
  • Maize Dwarf Mosaic Virus (MDMV)
  • Megasphaera cerevisiae / Megasphaera elsdenii (M.cerevisiae / M.elsdenii)
  • Yellow Mealworm Beetle (T.molitor)
  • Candidatus Liberibacter asiaticus (C.L.asiaticus )
  • Dekkera bruxellensis (D.bruxellensis)
  • Maize Mosaic Virus (MMV)
  • Potato mop-top virus (PMTV)
  • Pectinatus species
  • Pediococcus species
  • Spiroplasma citri (S.citri)
  • Saccharomyces cerevisiae var. diastaticus (S.diastaticus)
  • Spongospora subterranea (S.subterranea)
  • Bacillus cereus E33 (B.cereus E33)

Pathogen Contamination

  • E.coli Shiga toxin (stx2A)
  • E.coli Shiga toxin (stx2C)
  • Shewanella putrefaciens

Speciation

  • European Eel (Anguilla anguilla)
  • European Plaice (Pleuronectes platessa)
  • House Mouse (Mus musculus)
  • Ostrich (Struthio camelus)
  • Pea (Pisum sativum)
  • Universal Fish

If you are interested in any OnDemand Kits please check out the purchasing section above, or alternatively contact us via email: enquiry@primerdesign.co.uk or telephone: +44 (0)2380 748 830.

contact your sales  or Customer Care representative for an OnDemand Design Request Form